Should you encounter any unexpected behaviour,
please let us know.

* T70N-F3L *

B-factor analysis for ID 2504011331561597345

Overall B-factor prediction

Correlation= 0.628 for 130 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA LYS 1 0.0063 15.3960 18.2500
26 CA VAL 2 0.0062 15.3563 14.6600
42 CA LEU 3 0.0032 13.5905 12.2900
61 CA GLU 4 0.0069 15.8084 16.5500
76 CA ARG 5 0.0036 13.7983 13.7000
100 CA CYS 6 0.0039 13.9893 12.4200
110 CA GLU 7 0.0040 14.0724 12.0700
125 CA LEU 8 0.0018 12.7104 10.9000
144 CA ALA 9 0.0020 12.8298 11.3600
154 CA ARG 10 0.0038 13.9421 12.2600
178 CA THR 11 0.0026 13.2108 11.6000
192 CA LEU 12 0.0017 12.6622 11.1800
211 CA LYS 13 0.0038 13.9554 11.9700
233 CA ARG 14 0.0096 17.3890 12.9600
257 CA LEU 15 0.0040 14.0807 11.7100
276 CA GLY 16 0.0048 14.5047 13.4800
283 CA MET 17 0.0021 12.9193 11.6000
300 CA ASP 18 0.0038 13.9085 14.4100
312 CA GLY 19 0.0079 16.3643 15.0500
319 CA TYR 20 0.0038 13.9399 11.9500
340 CA ARG 21 0.0071 15.9159 14.2800
364 CA GLY 22 0.0259 27.1270 12.7600
371 CA ILE 23 0.0032 13.5595 11.7700
390 CA SER 24 0.0037 13.8777 11.9700
401 CA LEU 25 0.0021 12.9138 12.2300
420 CA ALA 26 0.0024 13.0756 12.1600
430 CA ASN 27 0.0018 12.7518 11.7200
444 CA TRP 28 0.0012 12.3992 10.7700
468 CA MET 29 0.0014 12.4852 10.6100
485 CA CYS 30 0.0018 12.7132 11.4700
495 CA LEU 31 0.0015 12.5606 12.2700
514 CA ALA 32 0.0016 12.6167 11.1200
524 CA LYS 33 0.0027 13.2935 12.8800
546 CA TRP 34 0.0048 14.5165 13.4600
570 CA GLU 35 0.0025 13.1486 13.1100
585 CA SER 36 0.0030 13.4504 13.2500
596 CA GLY 37 0.0041 14.1303 14.6200
603 CA TYR 38 0.0025 13.1782 12.4100
624 CA ASN 39 0.0035 13.7429 13.0000
638 CA THR 40 0.0029 13.3831 12.6800
652 CA ARG 41 0.0054 14.8723 16.4600
676 CA ALA 42 0.0040 14.0628 15.2400
686 CA THR 43 0.0059 15.1933 16.6300
700 CA ASN 44 0.0107 18.0688 17.3600
714 CA TYR 45 0.0128 19.2685 19.2600
735 CA ASN 46 0.0155 20.8794 18.0700
749 CA ALA 47 0.0331 31.4007 23.8000
759 CA GLY 48 0.0620 48.6221 22.0700
766 CA ASP 49 0.0215 24.5101 20.3500
778 CA ARG 50 0.0137 19.8296 19.6800
802 CA SER 51 0.0060 15.2341 15.6200
813 CA THR 52 0.0040 14.0220 11.9400
827 CA ASP 53 0.0032 13.6043 12.5900
839 CA TYR 54 0.0025 13.1309 11.1000
860 CA GLY 55 0.0022 12.9948 11.2200
867 CA ILE 56 0.0018 12.7170 11.0600
886 CA PHE 57 0.0015 12.5454 11.5800
906 CA GLN 58 0.0023 13.0515 11.5900
923 CA ILE 59 0.0017 12.7076 12.6900
942 CA ASN 60 0.0028 13.3646 12.2300
956 CA SER 61 0.0025 13.1513 12.0900
967 CA ARG 62 0.0053 14.7977 16.6100
991 CA TYR 63 0.0052 14.7855 17.9700
1012 CA TRP 64 0.0025 13.1454 13.1500
1036 CA CYS 65 0.0023 13.0641 12.6000
1046 CA ASN 66 0.0044 14.3190 12.0700
1060 CA ASP 67 0.0065 15.5510 13.4200
1072 CA GLY 68 0.0266 27.4961 14.7100
1079 CA LYS 69 0.0152 20.7466 17.6000
1101 CA ASN 70 0.0078 16.3151 18.0100
1115 CA PRO 71 0.0209 24.1452 23.9600
1129 CA GLY 72 0.0353 32.7158 31.9800
1136 CA ALA 73 0.0086 16.7765 26.1000
1146 CA VAL 74 0.0115 18.5445 27.9900
1162 CA ASN 75 0.0056 14.9997 17.5800
1176 CA ALA 76 0.0058 15.1188 15.8400
1186 CA CYS 77 0.0037 13.8959 15.1500
1196 CA HIS 78 0.0128 19.3123 16.7100
1213 CA LEU 79 0.0046 14.4291 16.1400
1232 CA SER 80 0.0062 15.3691 13.2300
1243 CA CYS 81 0.0029 13.3763 11.8200
1253 CA SER 82 0.0064 15.4762 13.5400
1264 CA ALA 83 0.0045 14.3592 12.5600
1274 CA LEU 84 0.0022 12.9747 12.7100
1293 CA LEU 85 0.0039 13.9827 13.5400
1312 CA GLN 86 0.0053 14.8447 14.0100
1329 CA ASP 87 0.0067 15.6804 14.6100
1341 CA ASN 88 0.0074 16.0575 17.7900
1355 CA ILE 89 0.0024 13.0917 12.7000
1374 CA ALA 90 0.0046 14.4118 11.8800
1384 CA ASP 91 0.0037 13.9024 13.0600
1396 CA ALA 92 0.0019 12.8146 12.9200
1406 CA VAL 93 0.0021 12.8991 11.5800
1422 CA ALA 94 0.0028 13.3277 14.0300
1432 CA CYS 95 0.0021 12.9440 11.4600
1442 CA ALA 96 0.0017 12.6806 11.0900
1452 CA LYS 97 0.0025 13.1651 11.6700
1474 CA ARG 98 0.0033 13.6137 12.8700
1498 CA VAL 99 0.0022 13.0045 12.3300
1514 CA VAL 100 0.0026 13.2279 12.6500
1530 CA ARG 101 0.0053 14.8046 17.7800
1554 CA ASP 102 0.0069 15.7851 18.6000
1566 CA PRO 103 0.0216 24.5318 24.3900
1580 CA GLN 104 0.0102 17.7652 21.2300
1597 CA GLY 105 0.0043 14.2299 17.9100
1604 CA ILE 106 0.0025 13.1529 17.1100
1623 CA ARG 107 0.0051 14.6926 17.1300
1647 CA ALA 108 0.0048 14.5151 21.5800
1657 CA TRP 109 0.0033 13.6062 16.7900
1681 CA VAL 110 0.0090 17.0525 20.7700
1697 CA ALA 111 0.0054 14.8752 20.0800
1707 CA TRP 112 0.0033 13.6221 14.2700
1731 CA ARG 113 0.0068 15.7010 16.4300
1755 CA ASN 114 0.0106 17.9662 17.9900
1769 CA ARG 115 0.0064 15.4719 17.3800
1793 CA CYS 116 0.0040 14.0503 14.6700
1803 CA GLN 117 0.0076 16.2168 18.2000
1820 CA ASN 118 0.0289 28.8753 28.5500
1834 CA ARG 119 0.0136 19.7757 29.4100
1858 CA ASP 120 0.0218 24.6672 27.3500
1870 CA VAL 121 0.0048 14.5035 21.1300
1886 CA ARG 122 0.0100 17.6105 27.6500
1910 CA GLN 123 0.0080 16.4484 28.1900
1927 CA TYR 124 0.0029 13.3805 20.5700
1948 CA VAL 125 0.0035 13.7693 19.0400
1964 CA GLN 126 0.0117 18.6587 29.0600
1981 CA GLY 127 0.0317 30.5713 25.3300
1988 CA CYS 128 0.0071 15.9292 20.3700
1998 CA GLY 129 0.0242 26.1087 29.2900
2005 CA VAL 130 0.0081 16.5014 25.2700

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: April 8th, 2025.

Should you encounter any unexpected behaviour, please let us know.