Should you encounter any unexpected behaviour,
please let us know.

* 1JSF_Native *

B-factor analysis for ID 2503180600413132399

Overall B-factor prediction

Correlation= 0.530 for 130 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA LYS 1 0.0257 15.3084 18.2500
11 CA VAL 2 0.0252 15.2443 14.6600
18 CA PHE 3 0.0146 13.8006 12.2900
29 CA GLU 4 0.0308 15.9990 16.5500
38 CA ARG 5 0.0164 14.0455 13.7000
49 CA CYS 6 0.0164 14.0476 12.4200
55 CA GLU 7 0.0187 14.3490 12.0700
64 CA LEU 8 0.0096 13.1122 10.9000
72 CA ALA 9 0.0097 13.1259 11.3600
77 CA ARG 10 0.0156 13.9344 12.2600
88 CA THR 11 0.0140 13.7111 11.6000
95 CA LEU 12 0.0094 13.0816 11.1800
103 CA LYS 13 0.0150 13.8550 11.9700
112 CA ARG 14 0.0410 17.3895 12.9600
130 CA LEU 15 0.0180 14.2662 11.7100
138 CA GLY 16 0.0234 14.9964 13.4800
142 CA MET 17 0.0106 13.2478 11.6000
150 CA ASP 18 0.0160 13.9876 14.4100
158 CA GLY 19 0.0265 15.4142 15.0500
162 CA TYR 20 0.0174 14.1822 11.9500
174 CA ARG 21 0.0305 15.9567 14.2800
185 CA GLY 22 0.1728 35.3462 12.7600
189 CA ILE 23 0.0148 13.8177 11.7700
197 CA SER 24 0.0148 13.8283 11.9700
203 CA LEU 25 0.0088 13.0002 12.2300
215 CA ALA 26 0.0090 13.0324 12.1600
220 CA ASN 27 0.0077 12.8589 11.7200
228 CA TRP 28 0.0060 12.6243 10.7700
242 CA MET 29 0.0057 12.5899 10.6100
250 CA CYS 30 0.0073 12.8026 11.4700
256 CA LEU 31 0.0065 12.6924 12.2700
264 CA ALA 32 0.0078 12.8687 11.1200
269 CA LYS 33 0.0109 13.2952 12.8800
278 CA TRP 34 0.0180 14.2561 13.4600
292 CA GLU 35 0.0108 13.2731 13.1100
301 CA SER 36 0.0127 13.5347 13.2500
307 CA GLY 37 0.0162 14.0180 14.6200
311 CA TYR 38 0.0132 13.6059 12.4100
323 CA ASN 39 0.0158 13.9637 13.0000
331 CA THR 40 0.0132 13.5993 12.6800
338 CA ARG 41 0.0206 14.6123 16.4600
356 CA ALA 42 0.0149 13.8393 15.2400
361 CA THR 43 0.0241 15.0884 16.6300
368 CA ASN 44 0.0388 17.0919 17.3600
376 CA TYR 45 0.0508 18.7271 19.2600
388 CA ASN 46 0.0557 19.3973 18.0700
396 CA ALA 47 0.1345 30.1265 23.8000
401 CA GLY 48 0.2323 43.4512 22.0700
405 CA ASP 49 0.0786 22.5182 20.3500
413 CA ARG 50 0.0512 18.7843 19.6800
424 CA SER 51 0.0236 15.0231 15.6200
430 CA THR 52 0.0141 13.7321 11.9400
437 CA ASP 53 0.0121 13.4556 12.5900
445 CA TYR 54 0.0093 13.0809 11.1000
457 CA GLY 55 0.0096 13.1214 11.2200
461 CA ILE 56 0.0082 12.9227 11.0600
469 CA PHE 57 0.0073 12.7986 11.5800
480 CA GLN 58 0.0104 13.2271 11.5900
489 CA ILE 59 0.0075 12.8347 12.6900
497 CA ASN 60 0.0112 13.3366 12.2300
505 CA SER 61 0.0095 13.1018 12.0900
511 CA ARG 62 0.0185 14.3248 16.6100
522 CA TYR 63 0.0207 14.6226 17.9700
534 CA TRP 64 0.0106 13.2551 13.1500
548 CA CYS 65 0.0097 13.1350 12.6000
554 CA ASN 66 0.0162 14.0155 12.0700
562 CA ASP 67 0.0247 15.1751 13.4200
570 CA GLY 68 0.1874 37.3359 14.7100
574 CA LYS 69 0.0631 20.4019 17.6000
583 CA THR 70 0.0313 16.0721 18.0100
590 CA PRO 71 0.0723 21.6574 23.9600
597 CA GLY 72 0.1672 34.5851 31.9800
601 CA ALA 73 0.0337 16.3993 26.1000
606 CA VAL 74 0.0436 17.7452 27.9900
613 CA ASN 75 0.0218 14.7739 17.5800
621 CA ALA 76 0.0224 14.8595 15.8400
626 CA CYS 77 0.0176 14.2109 15.1500
632 CA HIS 78 0.0516 18.8394 16.7100
642 CA LEU 79 0.0197 14.4909 16.1400
650 CA SER 80 0.0236 15.0270 13.2300
656 CA CYS 81 0.0129 13.5636 11.8200
662 CA SER 82 0.0256 15.2959 13.5400
670 CA ALA 83 0.0212 14.6971 12.5600
675 CA LEU 84 0.0099 13.1624 12.7100
683 CA LEU 85 0.0165 14.0584 13.5400
691 CA GLN 86 0.0231 14.9548 14.0100
700 CA ASP 87 0.0292 15.7847 14.6100
708 CA ASN 88 0.0358 16.6781 17.7900
716 CA ILE 89 0.0141 13.7290 12.7000
724 CA ALA 90 0.0291 15.7716 11.8800
729 CA ASP 91 0.0208 14.6432 13.0600
737 CA ALA 92 0.0112 13.3280 12.9200
742 CA VAL 93 0.0120 13.4473 11.5800
749 CA ALA 94 0.0159 13.9719 14.0300
754 CA CYS 95 0.0107 13.2592 11.4600
760 CA ALA 96 0.0092 13.0585 11.0900
765 CA LYS 97 0.0120 13.4391 11.6700
774 CA ARG 98 0.0154 13.9015 12.8700
785 CA VAL 99 0.0113 13.3503 12.3300
792 CA VAL 100 0.0123 13.4764 12.6500
799 CA ARG 101 0.0225 14.8682 17.7800
810 CA ASP 102 0.0296 15.8426 18.6000
818 CA PRO 103 0.0831 23.1221 24.3900
825 CA GLN 104 0.0455 18.0090 21.2300
834 CA GLY 105 0.0225 14.8661 17.9100
838 CA ILE 106 0.0123 13.4882 17.1100
846 CA ARG 107 0.0235 15.0109 17.1300
857 CA ALA 108 0.0237 15.0420 21.5800
862 CA TRP 109 0.0160 13.9866 16.7900
876 CA VAL 110 0.0371 16.8671 20.7700
883 CA ALA 111 0.0215 14.7386 20.0800
888 CA TRP 112 0.0145 13.7857 14.2700
902 CA ARG 113 0.0261 15.3571 16.4300
913 CA ASN 114 0.0430 17.6587 17.9900
921 CA ARG 115 0.0244 15.1257 17.3800
932 CA CYS 116 0.0161 13.9971 14.6700
938 CA GLN 117 0.0308 15.9979 18.2000
947 CA ASN 118 0.0987 25.2530 28.5500
955 CA ARG 119 0.0531 19.0396 29.4100
966 CA ASP 120 0.0807 22.7955 27.3500
974 CA VAL 121 0.0185 14.3320 21.1300
981 CA ARG 122 0.0280 15.6157 27.6500
999 CA GLN 123 0.0260 15.3445 28.1900
1008 CA TYR 124 0.0117 13.4031 20.5700
1020 CA VAL 125 0.0148 13.8196 19.0400
1027 CA GLN 126 0.0377 16.9406 29.0600
1041 CA GLY 127 0.1540 32.7771 25.3300
1045 CA CYS 128 0.0280 15.6153 20.3700
1051 CA GLY 129 0.0831 23.1281 29.2900
1055 CA VAL 130 0.0311 16.0413 25.2700

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: April 8th, 2025.

Should you encounter any unexpected behaviour, please let us know.