Should you encounter any unexpected behaviour,
please let us know.

* CYTOKINE/SIGNALING PROTEIN 21-APR-16 5JHW *

B-factor analysis for ID 2502060540332303681

Overall B-factor prediction

Correlation= 0.309 for 218 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA ASN 1 0.0472 79.4771 77.9300
17 CA LEU 2 0.0248 57.4945 65.0200
36 CA GLY 3 0.0116 44.6186 61.8100
43 CA LEU 4 0.0100 42.9861 47.1800
62 CA ASP 5 0.0099 42.8645 47.2500
74 CA CYS 6 0.0101 43.1287 51.2000
84 CA ASP 7 0.0156 48.4806 65.7900
96 CA GLU 8 0.0201 52.9352 80.7700
111 CA HIS 9 0.0329 65.4941 90.3800
128 CA SER 10 0.0289 61.5866 70.7400
139 CA SER 11 0.0414 73.8361 53.0000
150 CA GLU 12 0.0182 51.0770 44.1400
165 CA SER 13 0.0158 48.6892 42.9400
176 CA ARG 14 0.0098 42.8332 40.7700
200 CA CYS 15 0.0059 38.9992 40.3700
210 CA CYS 16 0.0060 39.1147 41.5500
220 CA ARG 17 0.0053 38.4117 36.4000
244 CA TYR 18 0.0058 38.8514 36.8200
265 CA PRO 19 0.0062 39.3143 36.9600
279 CA LEU 20 0.0050 38.1237 44.9900
298 CA THR 21 0.0068 39.8244 48.4300
312 CA VAL 22 0.0049 37.9672 44.4600
328 CA ASP 23 0.0087 41.7061 47.0600
340 CA PHE 24 0.0074 40.4736 46.9000
360 CA GLU 25 0.0158 48.7194 72.3000
375 CA ALA 26 0.0207 53.4834 81.8100
385 CA PHE 27 0.0139 46.8624 81.4100
405 CA GLY 28 0.0210 53.8041 85.5800
412 CA TRP 29 0.0137 46.6497 78.2700
436 CA ASP 30 0.0197 52.5599 80.4800
448 CA TRP 31 0.0180 50.8690 65.6800
472 CA ILE 32 0.0109 43.9119 45.2300
491 CA ILE 33 0.0156 48.4832 41.0100
510 CA ALA 34 0.0132 46.1387 43.0900
520 CA PRO 35 0.0101 43.1461 41.3600
534 CA LYS 36 0.0107 43.7410 46.7500
556 CA ARG 37 0.0081 41.0997 50.3300
580 CA TYR 38 0.0066 39.6494 39.9900
601 CA LYS 39 0.0072 40.2510 43.7900
623 CA ALA 40 0.0039 37.0252 30.6700
633 CA ASN 41 0.0042 37.3650 39.0200
647 CA TYR 42 0.0041 37.2377 31.9000
668 CA CYS 43 0.0043 37.3757 31.9800
678 CA SER 44 0.0055 38.5466 32.1100
689 CA GLY 45 0.0081 41.1249 42.7300
696 CA GLN 46 0.0097 42.7356 41.7700
713 CA CYS 47 0.0069 39.9370 41.9800
723 CA GLU 48 0.0105 43.5086 54.3300
738 CA TYR 49 0.0136 46.5600 51.4500
759 CA MET 50 0.0095 42.4828 45.5800
776 CA PHE 51 0.0093 42.3480 39.1000
796 CA MET 52 0.0077 40.7656 38.3400
813 CA GLN 53 0.0059 38.9729 36.5600
830 CA LYS 54 0.0071 40.1272 44.5600
852 CA TYR 55 0.0053 38.4329 43.8000
873 CA PRO 56 0.0044 37.5430 34.4400
887 CA HIS 57 0.0032 36.3249 39.1900
904 CA THR 58 0.0036 36.6812 35.2600
918 CA HIS 59 0.0048 37.9066 35.7900
935 CA LEU 60 0.0037 36.8097 42.2300
954 CA VAL 61 0.0040 37.0898 46.1600
970 CA GLN 62 0.0050 38.0643 42.2900
987 CA GLN 63 0.0074 40.4322 51.3800
1004 CA ALA 64 0.0074 40.4308 53.8100
1014 CA ASN 65 0.0113 44.2396 55.6600
1028 CA PRO 66 0.0098 42.7643 56.2800
1042 CA ARG 67 0.0203 53.1431 67.3800
1066 CA GLY 68 0.0178 50.6401 55.3000
1073 CA SER 69 0.0078 40.8403 60.9100
1084 CA ALA 70 0.0094 42.3934 55.3300
1094 CA GLY 71 0.0084 41.4020 50.8300
1101 CA PRO 72 0.0057 38.7692 38.9500
1115 CA CYS 73 0.0045 37.6457 33.9500
1125 CA CYS 74 0.0044 37.5432 28.7000
1135 CA THR 75 0.0040 37.1049 34.7400
1149 CA PRO 76 0.0044 37.4720 35.4600
1163 CA THR 77 0.0068 39.8615 37.0400
1177 CA LYS 78 0.0082 41.2252 39.2700
1199 CA MET 79 0.0069 39.9456 39.3300
1216 CA SER 80 0.0078 40.8530 33.3500
1227 CA PRO 81 0.0094 42.4139 34.4900
1241 CA ILE 82 0.0079 40.9855 35.0700
1260 CA ASN 83 0.0129 45.8151 37.3700
1274 CA MET 84 0.0101 43.1208 35.9100
1291 CA LEU 85 0.0149 47.8662 36.3100
1310 CA TYR 86 0.0150 47.9284 35.7700
1331 CA PHE 87 0.0260 58.6771 39.8600
1351 CA ASN 88 0.0384 70.8446 42.0900
1365 CA ASP 89 0.0843 115.9591 51.0700
1377 CA LYS 90 0.0863 117.8616 42.8900
1399 CA GLN 91 0.0559 88.0937 46.6700
1416 CA GLN 92 0.0526 84.7687 41.1100
1433 CA ILE 93 0.0346 67.1767 37.0500
1452 CA ILE 94 0.0297 62.3146 33.1500
1471 CA TYR 95 0.0237 56.4222 38.8700
1492 CA GLY 96 0.0202 52.9967 34.6500
1499 CA LYS 97 0.0164 49.3230 42.6700
1521 CA ILE 98 0.0107 43.6539 29.7100
1540 CA PRO 99 0.0099 42.9122 33.7200
1554 CA GLY 100 0.0064 39.5077 26.7500
1561 CA MET 101 0.0042 37.3243 35.1100
1578 CA VAL 102 0.0048 37.8595 41.7700
1594 CA VAL 103 0.0047 37.8427 38.1800
1610 CA ASP 104 0.0067 39.7231 34.0500
1622 CA ARG 105 0.0064 39.4484 35.1300
1646 CA CYS 106 0.0051 38.1930 33.4700
1656 CA GLY 107 0.0050 38.1230 34.0400
1663 CA CYS 108 0.0046 37.7543 34.9700
1673 CA SER 109 0.0042 37.2755 50.5900
1685 CA ASN 1 0.0366 69.0910 68.0400
1700 CA LEU 2 0.0208 53.6496 53.6100
1719 CA GLY 3 0.0106 43.6078 56.4000
1726 CA LEU 4 0.0097 42.6987 44.2600
1745 CA ASP 5 0.0098 42.8483 48.2400
1757 CA CYS 6 0.0103 43.2673 53.5500
1767 CA ASP 7 0.0207 53.4683 69.8700
1779 CA GLU 8 0.0375 69.9517 82.7800
1794 CA HIS 9 0.0500 82.2976 86.4900
1811 CA SER 10 0.0358 68.3720 75.6700
1822 CA SER 11 0.0455 77.8338 73.7600
1833 CA GLU 12 0.0187 51.5066 57.2000
1848 CA SER 13 0.0156 48.4806 56.2000
1859 CA ARG 14 0.0097 42.7522 48.7000
1883 CA CYS 15 0.0058 38.9318 45.7700
1893 CA CYS 16 0.0060 39.0871 35.0100
1903 CA ARG 17 0.0053 38.3717 32.6500
1927 CA TYR 18 0.0058 38.8420 35.7700
1948 CA PRO 19 0.0065 39.5771 36.1700
1962 CA LEU 20 0.0051 38.2421 35.0700
1981 CA THR 21 0.0070 40.0617 40.6500
1995 CA VAL 22 0.0050 38.0550 48.8700
2011 CA ASP 23 0.0088 41.7858 52.1000
2023 CA PHE 24 0.0073 40.3498 40.4600
2043 CA GLU 25 0.0155 48.4185 64.9500
2058 CA ALA 26 0.0201 52.9314 57.7300
2068 CA PHE 27 0.0130 45.9696 72.2000
2088 CA GLY 28 0.0196 52.3843 80.5800
2095 CA TRP 29 0.0128 45.7379 73.1900
2119 CA ASP 30 0.0184 51.2500 67.5100
2131 CA TRP 31 0.0174 50.2228 51.2400
2155 CA ILE 32 0.0105 43.5147 40.7800
2174 CA ILE 33 0.0153 48.1627 40.7800
2193 CA ALA 34 0.0130 45.9240 36.0000
2203 CA PRO 35 0.0102 43.1804 39.0600
2217 CA LYS 36 0.0110 43.9974 46.0200
2239 CA ARG 37 0.0086 41.6253 47.2400
2263 CA TYR 38 0.0068 39.9153 39.9600
2284 CA LYS 39 0.0075 40.6003 38.1400
2306 CA ALA 40 0.0039 37.0278 32.2400
2316 CA ASN 41 0.0043 37.4055 29.8700
2330 CA TYR 42 0.0041 37.2337 30.8600
2351 CA CYS 43 0.0043 37.3722 33.2600
2361 CA SER 44 0.0055 38.5841 34.9200
2372 CA GLY 45 0.0082 41.2631 42.1000
2379 CA GLN 46 0.0098 42.7679 47.7100
2396 CA CYS 47 0.0070 40.0289 46.3900
2406 CA GLU 48 0.0103 43.2821 50.4400
2421 CA TYR 49 0.0137 46.6619 45.3700
2442 CA MET 50 0.0101 43.1217 47.3600
2459 CA PHE 51 0.0095 42.5008 38.7700
2479 CA MET 52 0.0079 40.9032 41.4300
2496 CA GLN 53 0.0058 38.9306 38.9700
2513 CA LYS 54 0.0069 39.9709 45.6200
2535 CA TYR 55 0.0054 38.5043 47.4800
2556 CA PRO 56 0.0046 37.6667 40.1800
2570 CA HIS 57 0.0033 36.4355 41.8600
2587 CA THR 58 0.0036 36.7755 43.1600
2601 CA HIS 59 0.0049 38.0457 46.5400
2618 CA LEU 60 0.0038 36.9461 53.1700
2637 CA VAL 61 0.0040 37.1252 56.6600
2653 CA GLN 62 0.0050 38.0871 51.4200
2670 CA GLN 63 0.0075 40.5228 58.2900
2687 CA ALA 64 0.0074 40.4643 65.3600
2697 CA ASN 65 0.0105 43.4954 68.0500
2711 CA PRO 66 0.0093 42.3706 67.4100
2725 CA ARG 67 0.0175 50.4007 82.4000
2749 CA GLY 68 0.0141 47.0137 77.5500
2756 CA SER 69 0.0081 41.1354 77.7700
2767 CA ALA 70 0.0108 43.7802 77.6600
2777 CA GLY 71 0.0067 39.7795 65.5900
2784 CA PRO 72 0.0053 38.4376 42.2300
2798 CA CYS 73 0.0044 37.5583 45.4000
2808 CA CYS 74 0.0044 37.4644 36.2600
2818 CA THR 75 0.0040 37.1006 39.4200
2832 CA PRO 76 0.0044 37.4803 30.0600
2846 CA THR 77 0.0070 40.0278 37.3100
2860 CA LYS 78 0.0083 41.2978 40.5400
2882 CA MET 79 0.0069 39.9411 42.8300
2899 CA SER 80 0.0078 40.8637 44.6100
2910 CA PRO 81 0.0095 42.4692 36.6200
2924 CA ILE 82 0.0080 41.0707 36.6100
2943 CA ASN 83 0.0128 45.7194 35.9200
2957 CA MET 84 0.0101 43.0688 32.9800
2974 CA LEU 85 0.0149 47.8569 30.3600
2993 CA TYR 86 0.0149 47.8074 31.7300
3014 CA PHE 87 0.0255 58.2215 40.1200
3034 CA ASN 88 0.0379 70.4193 42.0800
3048 CA ASP 89 0.0812 112.8424 52.9100
3060 CA LYS 90 0.0850 116.5948 50.9000
3082 CA GLN 91 0.0559 88.0906 44.7600
3099 CA GLN 92 0.0525 84.7086 40.4400
3116 CA ILE 93 0.0344 66.9655 39.2800
3135 CA ILE 94 0.0296 62.2834 39.3400
3154 CA TYR 95 0.0235 56.2813 34.3500
3175 CA GLY 96 0.0206 53.4591 36.2500
3182 CA LYS 97 0.0161 49.0390 41.7800
3204 CA ILE 98 0.0106 43.6212 34.9000
3223 CA PRO 99 0.0097 42.6931 31.9600
3237 CA GLY 100 0.0063 39.3881 29.9300
3244 CA MET 101 0.0041 37.2651 35.1400
3261 CA VAL 102 0.0047 37.8218 31.9000
3277 CA VAL 103 0.0047 37.8129 33.2100
3293 CA ASP 104 0.0066 39.7141 34.4400
3305 CA ARG 105 0.0063 39.4223 35.8700
3329 CA CYS 106 0.0051 38.2240 33.0100
3339 CA GLY 107 0.0050 38.0725 28.9000
3346 CA CYS 108 0.0046 37.7247 34.3100
3356 CA SER 109 0.0042 37.3202 51.7000

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: April 8th, 2025.

Should you encounter any unexpected behaviour, please let us know.