Should you encounter any unexpected behaviour,
please let us know.

* gfp_th *

B-factor analysis for ID 2502030431161368890

Overall B-factor prediction

Correlation= 0.428 for 239 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA LYS 3 0.0083 7.8313 33.0700
26 CA GLY 4 0.0040 6.3136 13.7300
33 CA GLU 5 0.0030 5.9580 13.8100
48 CA GLU 6 0.0073 7.4716 14.0100
63 CA LEU 7 0.0033 6.0403 9.1100
82 CA PHE 8 0.0026 5.8074 7.6100
102 CA THR 9 0.0079 7.7199 9.5000
116 CA GLY 10 0.0078 7.6527 9.4800
123 CA VAL 11 0.0050 6.6693 7.4600
139 CA VAL 12 0.0032 6.0162 5.9200
155 CA PRO 13 0.0028 5.8634 5.8400
169 CA ILE 14 0.0018 5.5070 5.4100
188 CA LEU 15 0.0018 5.5264 4.5000
189 CA LEU 15 0.0018 5.5275 5.2900
222 CA VAL 16 0.0015 5.3999 4.6900
238 CA GLU 17 0.0015 5.4003 4.2700
253 CA LEU 18 0.0014 5.3647 5.1100
285 CA ASP 19 0.0024 5.7313 5.6400
297 CA GLY 20 0.0022 5.6628 5.9700
304 CA ASP 21 0.0031 5.9628 4.9200
316 CA VAL 22 0.0023 5.7017 4.7200
332 CA ASN 23 0.0047 6.5589 5.2100
346 CA GLY 24 0.0089 8.0775 6.3600
353 CA HIS 25 0.0062 7.0880 6.5900
371 CA LYS 26 0.0079 7.6989 7.5800
393 CA PHE 27 0.0025 5.7722 5.4500
413 CA SER 28 0.0024 5.7139 5.4000
424 CA VAL 29 0.0014 5.3828 4.7900
440 CA SER 30 0.0016 5.4582 5.1300
456 CA GLY 31 0.0016 5.4373 5.5000
463 CA GLU 32 0.0020 5.5929 4.9200
478 CA GLY 33 0.0026 5.8111 6.7800
485 CA GLU 34 0.0023 5.6814 6.0700
500 CA GLY 35 0.0021 5.6051 6.3500
507 CA ASP 36 0.0025 5.7540 8.7800
519 CA ALA 37 0.0020 5.5779 6.0800
529 CA THR 38 0.0040 6.2884 7.9800
543 CA TYR 39 0.0045 6.4866 7.4800
564 CA GLY 40 0.0023 5.7035 5.7100
571 CA LYS 41 0.0023 5.6763 5.2000
594 CA LEU 42 0.0019 5.5401 5.4700
595 CA LEU 42 0.0018 5.5294 4.7700
632 CA THR 43 0.0021 5.6047 5.0100
633 CA THR 43 0.0021 5.6146 7.0600
659 CA LEU 44 0.0019 5.5567 5.3800
678 CA LYS 45 0.0017 5.4927 4.8700
700 CA PHE 46 0.0013 5.3437 5.0600
720 CA ILE 47 0.0022 5.6603 5.2000
748 CA CYS 48 0.0023 5.6809 5.2400
758 CA THR 49 0.0041 6.3317 8.9200
772 CA THR 50 0.0066 7.2343 9.7400
786 CA GLY 51 0.0085 7.9011 11.9400
793 CA LYS 52 0.0071 7.4147 10.1900
815 CA LEU 53 0.0020 5.6027 6.4100
834 CA PRO 54 0.0026 5.7863 6.5900
848 CA VAL 55 0.0014 5.3700 5.9900
864 CA PRO 56 0.0016 5.4262 4.7500
878 CA TRP 57 0.0011 5.2457 4.0100
902 CA PRO 58 0.0013 5.3341 3.8800
916 CA THR 59 0.0010 5.2133 3.8000
930 CA LEU 60 0.0010 5.2155 3.7800
949 CA VAL 61 0.0011 5.2531 3.5100
965 CA THR 62 0.0011 5.2796 3.4600
979 CA THR 63 0.0011 5.2659 3.9200
993 CA LEU 64 0.0010 5.2405 3.5000
1012 CA VAL 68 0.0008 5.1703 4.3300
1028 CA GLN 69 0.0008 5.1414 4.4700
1045 CA CYS 70 0.0011 5.2619 4.8900
1055 CA PHE 71 0.0011 5.2739 4.6900
1075 CA SER 72 0.0016 5.4441 5.3700
1086 CA ARG 73 0.0022 5.6455 4.7500
1087 CA ARG 73 0.0022 5.6567 6.0000
1130 CA TYR 74 0.0022 5.6519 5.8500
1151 CA PRO 75 0.0036 6.1591 7.1000
1165 CA ASP 76 0.0116 9.0212 7.0900
1177 CA HIS 77 0.0118 9.0928 10.3100
1194 CA MET 78 0.0035 6.1388 9.4300
1222 CA LYS 79 0.0035 6.1067 8.5000
1244 CA ARG 80 0.0044 6.4348 7.1000
1268 CA HIS 81 0.0024 5.7361 5.8400
1285 CA ASP 82 0.0014 5.3627 5.7700
1297 CA PHE 83 0.0011 5.2769 5.6900
1317 CA PHE 84 0.0010 5.2192 5.4600
1337 CA LYS 85 0.0011 5.2702 5.7800
1359 CA SER 86 0.0020 5.5726 7.3800
1360 CA SER 86 0.0020 5.5711 7.3200
1377 CA ALA 87 0.0017 5.4915 7.7000
1387 CA MET 88 0.0021 5.6148 7.3900
1404 CA PRO 89 0.0026 5.8124 8.3800
1419 CA GLU 90 0.0040 6.2920 9.2200
1420 CA GLU 90 0.0039 6.2829 8.5300
1448 CA GLY 91 0.0021 5.6126 8.0800
1455 CA TYR 92 0.0013 5.3286 5.8500
1476 CA VAL 93 0.0016 5.4249 5.8900
1492 CA GLN 94 0.0014 5.3685 4.3300
1509 CA GLU 95 0.0016 5.4536 5.3200
1524 CA ARG 96 0.0016 5.4418 4.1700
1548 CA THR 97 0.0019 5.5657 4.8300
1562 CA ILE 98 0.0016 5.4408 4.3500
1581 CA PHE 99 0.0022 5.6411 4.5300
1601 CA PHE 100 0.0021 5.6305 4.5400
1621 CA LYS 101 0.0040 6.2896 4.9800
1643 CA ASP 102 0.0105 8.6243 7.1100
1655 CA ASP 103 0.0040 6.3157 5.2000
1667 CA GLY 104 0.0028 5.8615 6.0100
1674 CA ASN 105 0.0024 5.7194 4.4800
1688 CA TYR 106 0.0016 5.4289 4.6300
1709 CA LYS 107 0.0025 5.7487 4.5200
1747 CA THR 108 0.0017 5.4645 4.7500
1761 CA ARG 109 0.0018 5.5309 5.2300
1785 CA ALA 110 0.0017 5.4865 5.1700
1795 CA GLU 111 0.0018 5.5260 6.2700
1810 CA VAL 112 0.0014 5.3790 5.6100
1826 CA LYS 113 0.0017 5.4883 6.2100
1848 CA PHE 114 0.0023 5.7008 6.7400
1868 CA GLU 115 0.0033 6.0519 7.4800
1883 CA GLY 116 0.0254 13.9913 9.3100
1890 CA ASP 117 0.0132 9.6185 8.9500
1902 CA THR 118 0.0027 5.8229 7.8300
1916 CA LEU 119 0.0017 5.4764 4.9400
1935 CA VAL 120 0.0017 5.4700 4.6600
1951 CA ASN 121 0.0014 5.3603 4.3600
1965 CA ARG 122 0.0017 5.4760 6.2500
2007 CA ILE 123 0.0018 5.5175 5.0500
2026 CA GLU 124 0.0025 5.7714 4.8400
2027 CA GLU 124 0.0026 5.7847 5.5000
2052 CA LEU 125 0.0018 5.5024 4.7200
2071 CA LYS 126 0.0027 5.8438 5.2400
2093 CA GLY 127 0.0028 5.8899 5.2000
2100 CA ILE 128 0.0041 6.3469 5.2500
2119 CA ASP 129 0.0059 6.9771 6.5600
2131 CA PHE 130 0.0036 6.1568 5.6500
2151 CA LYS 131 0.0054 6.8009 7.4800
2152 CA LYS 131 0.0054 6.7887 6.9900
2191 CA GLU 132 0.0134 9.6761 9.3600
2206 CA ASP 133 0.0171 11.0012 7.0500
2218 CA GLY 134 0.0044 6.4399 5.7000
2225 CA ASN 135 0.0033 6.0505 6.0200
2239 CA ILE 136 0.0021 5.6205 5.6800
2258 CA LEU 137 0.0030 5.9564 6.2100
2277 CA GLY 138 0.0048 6.6059 6.4400
2284 CA HIS 139 0.0039 6.2588 5.5600
2301 CA LYS 140 0.0052 6.7479 4.7500
2323 CA LEU 141 0.0027 5.8512 4.8700
2342 CA GLU 142 0.0038 6.2241 4.7800
2343 CA GLU 142 0.0038 6.2359 5.1000
2368 CA TYR 143 0.0027 5.8292 4.6500
2389 CA ASN 144 0.0028 5.8765 5.9000
2403 CA TYR 145 0.0022 5.6500 4.8200
2424 CA ASN 146 0.0032 6.0032 5.5300
2438 CA SER 147 0.0041 6.3271 5.2300
2449 CA HIS 148 0.0026 5.7959 4.6500
2466 CA ASN 149 0.0028 5.8822 5.2400
2480 CA VAL 150 0.0019 5.5651 4.9300
2496 CA TYR 151 0.0023 5.7037 4.8000
2517 CA ILE 152 0.0017 5.4845 5.7700
2536 CA MET 153 0.0022 5.6754 8.2100
2553 CA ALA 154 0.0031 5.9822 9.7000
2563 CA ASP 155 0.0031 5.9797 10.6400
2575 CA LYS 156 0.0084 7.8728 15.4000
2597 CA GLN 157 0.0148 10.1639 13.3000
2614 CA LYS 158 0.0045 6.4936 13.1100
2636 CA ASN 159 0.0040 6.2969 10.3800
2650 CA GLY 160 0.0019 5.5674 8.1300
2657 CA ILE 161 0.0016 5.4297 6.1200
2676 CA LYS 162 0.0020 5.5899 6.7000
2714 CA VAL 163 0.0018 5.5139 6.4100
2730 CA ASN 164 0.0020 5.5975 5.1900
2731 CA ASN 164 0.0020 5.5948 6.3800
2754 CA PHE 165 0.0020 5.5956 5.1500
2775 CA LYS 166 0.0027 5.8387 5.5800
2776 CA LYS 166 0.0028 5.8685 4.8100
2818 CA THR 167 0.0023 5.6776 4.7200
2832 CA ARG 168 0.0023 5.6925 4.2000
2856 CA HIS 169 0.0020 5.5904 3.9400
2873 CA ASN 170 0.0036 6.1735 4.1200
2887 CA ILE 171 0.0037 6.2075 4.6400
2906 CA GLU 172 0.0098 8.3675 5.7500
2921 CA ASP 173 0.0217 12.6582 6.4100
2933 CA GLY 174 0.0179 11.2930 6.8400
2940 CA SER 175 0.0159 10.5654 5.7900
2951 CA VAL 176 0.0040 6.3068 5.0200
2967 CA GLN 177 0.0027 5.8242 5.1500
2984 CA LEU 178 0.0026 5.7847 4.5500
3003 CA ALA 179 0.0020 5.5841 4.4800
3013 CA ASP 180 0.0022 5.6472 4.5900
3025 CA HIS 181 0.0018 5.5241 4.3100
3042 CA TYR 182 0.0019 5.5422 5.1400
3063 CA GLN 183 0.0016 5.4423 5.2400
3080 CA GLN 184 0.0019 5.5496 5.3000
3081 CA GLN 184 0.0019 5.5654 7.5900
3110 CA ASN 185 0.0015 5.3971 5.3000
3124 CA THR 186 0.0020 5.5795 7.2200
3146 CA PRO 187 0.0023 5.6891 6.9200
3160 CA ILE 188 0.0034 6.1030 8.3100
3179 CA GLY 189 0.0054 6.8212 12.6900
3186 CA ASP 190 0.0234 13.2540 12.5200
3187 CA ASP 190 0.0234 13.2670 12.5100
3206 CA GLY 191 0.0294 15.4006 11.4700
3213 CA PRO 192 0.0163 10.7173 9.7300
3227 CA VAL 193 0.0044 6.4488 9.3800
3243 CA LEU 194 0.0025 5.7598 12.4000
3262 CA LEU 195 0.0026 5.7853 8.0800
3281 CA PRO 196 0.0026 5.7919 7.3800
3295 CA ASP 197 0.0046 6.5241 10.1600
3307 CA ASN 198 0.0040 6.3058 8.1200
3321 CA HIS 199 0.0027 5.8398 5.6900
3338 CA TYR 200 0.0022 5.6591 5.5500
3359 CA LEU 201 0.0020 5.5853 5.4000
3378 CA SER 202 0.0028 5.8637 4.7300
3389 CA THR 203 0.0028 5.8596 4.8300
3403 CA GLN 204 0.0037 6.2015 5.9200
3420 CA SER 205 0.0031 5.9677 5.3900
3431 CA ALA 206 0.0026 5.8169 5.8600
3441 CA LEU 207 0.0022 5.6464 4.9100
3460 CA SER 208 0.0049 6.6162 5.2100
3471 CA LYS 209 0.0050 6.6767 6.3900
3493 CA ASP 210 0.0110 8.8001 6.9600
3505 CA PRO 211 0.0258 14.1321 8.0700
3519 CA ASN 212 0.0255 14.0190 6.1800
3533 CA GLU 213 0.0106 8.6801 7.3700
3548 CA LYS 214 0.0176 11.1721 9.9200
3586 CA ARG 215 0.0108 8.7432 6.2500
3610 CA ASP 216 0.0032 6.0082 5.3600
3622 CA HIS 217 0.0024 5.7307 4.1000
3639 CA MET 218 0.0015 5.4017 4.1100
3656 CA VAL 219 0.0018 5.5100 4.0200
3672 CA LEU 220 0.0020 5.5744 4.6100
3691 CA LEU 221 0.0022 5.6532 4.5500
3710 CA GLU 222 0.0022 5.6579 4.8100
3725 CA PHE 223 0.0027 5.8360 4.2800
3745 CA VAL 224 0.0020 5.5964 4.7300
3761 CA THR 225 0.0022 5.6524 5.7400
3775 CA ALA 226 0.0023 5.6942 5.3800
3785 CA ALA 227 0.0032 5.9989 6.3800
3795 CA GLY 228 0.0117 9.0504 11.7900
3802 CA ILE 229 0.0250 13.8222 15.2500
3821 CA THR 230 0.0531 23.9359 17.1700
3835 CA HIS 231 0.0941 38.6292 11.7700

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: April 8th, 2025.

Should you encounter any unexpected behaviour, please let us know.