Should you encounter any unexpected behaviour,
please let us know.

* 5_HT *

B-factor analysis for ID 22050518472498844

Overall B-factor prediction

Correlation= 0.392 for 370 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA SER 67 0.1007 135.5645 104.9000
15 CA LEU 68 0.0545 97.0755 128.0700
34 CA LEU 69 0.0381 83.4030 129.2800
53 CA HIS 70 0.0458 89.7704 132.1900
70 CA LEU 71 0.0393 84.3946 121.6500
89 CA GLN 72 0.0393 84.3993 128.6100
106 CA GLU 73 0.0449 89.0026 130.6400
121 CA LYS 74 0.0302 76.8005 138.6700
143 CA ASN 75 0.0325 78.6921 125.4600
157 CA TRP 76 0.0343 80.2183 100.9000
181 CA SER 77 0.0189 67.4006 97.1500
192 CA ALA 78 0.0186 67.1462 89.6500
202 CA LEU 79 0.0235 71.1874 102.6800
221 CA LEU 80 0.0188 67.2743 80.9100
240 CA THR 81 0.0094 59.4674 82.4300
254 CA ALA 82 0.0113 61.0617 82.1000
264 CA VAL 83 0.0115 61.1682 68.9800
280 CA VAL 84 0.0068 57.2671 77.2400
296 CA ILE 85 0.0057 56.4017 64.0800
315 CA ILE 86 0.0087 58.9048 71.2900
334 CA LEU 87 0.0074 57.7674 51.6300
353 CA THR 88 0.0047 55.5086 71.7800
367 CA ILE 89 0.0057 56.4048 69.4100
386 CA ALA 90 0.0079 58.1713 77.0300
396 CA GLY 91 0.0066 57.1216 54.8600
403 CA ASN 92 0.0060 56.6257 60.4700
417 CA ILE 93 0.0081 58.3663 70.3600
436 CA LEU 94 0.0098 59.8008 58.6300
455 CA VAL 95 0.0091 59.2431 56.9000
471 CA ILE 96 0.0100 59.9347 62.5400
490 CA MET 97 0.0137 63.0188 70.7100
507 CA ALA 98 0.0147 63.8393 57.4200
517 CA VAL 99 0.0153 64.3636 63.3100
533 CA SER 100 0.0175 66.2257 61.0700
544 CA LEU 101 0.0231 70.8710 63.8400
563 CA GLU 102 0.0241 71.7161 64.8400
578 CA LYS 103 0.0339 79.8740 81.8700
600 CA LYS 104 0.0317 78.0594 85.9400
622 CA LEU 105 0.0223 70.2046 76.3700
641 CA GLN 106 0.0258 73.1268 73.1300
658 CA ASN 107 0.0232 70.9932 64.1700
672 CA ALA 108 0.0199 68.2430 85.5600
682 CA THR 109 0.0164 65.2694 71.0000
696 CA ASN 110 0.0148 63.9790 67.5300
710 CA TYR 111 0.0146 63.7639 70.7800
731 CA PHE 112 0.0117 61.3640 53.5700
751 CA LEU 113 0.0097 59.6871 52.8500
770 CA MET 114 0.0095 59.5107 60.6500
787 CA SER 115 0.0084 58.5904 49.5900
798 CA LEU 116 0.0062 56.7807 65.3300
817 CA ALA 117 0.0057 56.3969 64.6700
827 CA ILE 118 0.0060 56.6669 57.5500
846 CA ALA 119 0.0043 55.2179 74.5100
856 CA ASP 120 0.0033 54.3767 71.3000
868 CA MET 121 0.0044 55.3138 82.1600
885 CA LEU 122 0.0047 55.5825 57.9000
904 CA LEU 123 0.0030 54.1298 49.3900
923 CA GLY 124 0.0037 54.7356 56.6900
930 CA PHE 125 0.0059 56.5804 58.5100
950 CA LEU 126 0.0062 56.7994 42.3100
969 CA VAL 127 0.0044 55.2508 50.7400
985 CA MET 128 0.0042 55.0983 52.8500
1002 CA PRO 129 0.0074 57.8002 69.8500
1016 CA VAL 130 0.0085 58.7221 63.3400
1032 CA SER 131 0.0069 57.3395 59.1500
1043 CA MET 132 0.0093 59.3748 77.2800
1060 CA LEU 133 0.0140 63.3031 79.8600
1079 CA THR 134 0.0135 62.8441 82.9700
1093 CA ILE 135 0.0149 64.0813 87.9100
1112 CA LEU 136 0.0207 68.8474 78.4600
1131 CA TYR 137 0.0242 71.7928 73.4400
1152 CA GLY 138 0.0245 72.0102 61.4800
1159 CA TYR 139 0.0171 65.8438 72.1800
1180 CA ARG 140 0.0219 69.8896 77.0400
1204 CA TRP 141 0.0187 67.2241 67.1000
1228 CA PRO 142 0.0236 71.3276 68.8400
1242 CA LEU 143 0.0238 71.4798 65.5800
1261 CA PRO 144 0.0297 76.3498 57.6200
1275 CA SER 145 0.0244 71.9374 64.2500
1286 CA LYS 146 0.0225 70.3795 71.5900
1308 CA LEU 147 0.0175 66.2014 67.7900
1327 CA CYS 148 0.0124 61.9549 63.0600
1337 CA ALA 149 0.0109 60.6959 60.8600
1347 CA VAL 150 0.0109 60.7326 61.1200
1363 CA TRP 151 0.0072 57.5923 60.3700
1387 CA ILE 152 0.0055 56.2148 63.2800
1406 CA TYR 153 0.0059 56.5242 56.4800
1427 CA LEU 154 0.0050 55.7528 54.1000
1446 CA ASP 155 0.0028 53.9211 67.3800
1458 CA VAL 156 0.0030 54.1627 57.6000
1474 CA LEU 157 0.0043 55.2418 54.6900
1493 CA PHE 158 0.0034 54.4773 56.7500
1513 CA SER 159 0.0026 53.8210 61.6100
1524 CA THR 160 0.0042 55.1299 56.4000
1538 CA ALA 161 0.0052 55.9843 56.2300
1548 CA LYS 162 0.0046 55.4586 52.9900
1570 CA ILE 163 0.0061 56.6791 52.9400
1589 CA TRP 164 0.0083 58.5366 55.6200
1613 CA HIS 165 0.0085 58.6925 56.8100
1631 CA LEU 166 0.0091 59.2479 50.9100
1650 CA CYS 167 0.0116 61.3025 57.3400
1661 CA ALA 168 0.0135 62.8893 57.4800
1671 CA ILE 169 0.0142 63.4267 55.2000
1690 CA SER 170 0.0163 65.1846 65.4800
1701 CA LEU 171 0.0188 67.3288 54.0600
1720 CA ASP 172 0.0204 68.5932 74.0600
1732 CA ARG 173 0.0217 69.6850 73.2700
1756 CA TYR 174 0.0248 72.2937 52.1400
1777 CA VAL 175 0.0285 75.3511 56.9800
1793 CA ALA 176 0.0296 76.3242 76.5100
1803 CA ILE 177 0.0313 77.7374 79.5200
1822 CA GLN 178 0.0359 81.5809 107.1200
1839 CA ASN 179 0.0424 86.9801 125.4500
1853 CA PRO 180 0.0458 89.7775 129.3100
1867 CA ILE 181 0.0579 99.8442 153.7100
1886 CA HIS 182 0.0442 88.4817 212.7900
1903 CA HIS 183 0.0369 82.3900 202.2300
1920 CA SER 184 0.0472 90.9889 165.7400
1931 CA ARG 185 0.0478 91.4219 152.6500
1955 CA PHE 186 0.0351 80.9129 137.6100
1975 CA ASN 187 0.0337 79.7090 150.4500
1989 CA SER 188 0.0338 79.7620 100.9200
2000 CA ARG 189 0.0265 73.7330 93.9800
2024 CA THR 190 0.0283 75.2334 72.1500
2038 CA LYS 191 0.0273 74.3982 76.4000
2060 CA ALA 192 0.0210 69.0921 62.5900
2070 CA PHE 193 0.0197 68.0416 72.7300
2090 CA LEU 194 0.0230 70.7596 59.9100
2109 CA LYS 195 0.0173 66.0258 69.0200
2131 CA ILE 196 0.0136 62.9619 53.9400
2150 CA ILE 197 0.0173 66.0518 69.6800
2169 CA ALA 198 0.0179 66.5172 66.9900
2179 CA VAL 199 0.0114 61.1579 64.1200
2195 CA TRP 200 0.0098 59.7676 66.0100
2219 CA THR 201 0.0147 63.8439 72.5600
2233 CA ILE 202 0.0130 62.4477 63.5000
2252 CA SER 203 0.0081 58.3491 56.0200
2263 CA VAL 204 0.0099 59.8628 65.8600
2279 CA GLY 205 0.0124 61.9958 52.7000
2286 CA ILE 206 0.0093 59.3834 57.7200
2305 CA SER 207 0.0075 57.8347 78.8900
2316 CA MET 208 0.0119 61.5310 89.5400
2333 CA PRO 209 0.0123 61.8883 76.6100
2347 CA ILE 210 0.0093 59.3403 74.0700
2366 CA PRO 211 0.0131 62.5732 58.1100
2380 CA VAL 212 0.0187 67.2034 52.9700
2396 CA PHE 213 0.0186 67.0955 72.5400
2416 CA GLY 214 0.0148 63.9495 59.7900
2423 CA LEU 215 0.0206 68.7999 57.8300
2442 CA GLN 216 0.0285 75.3388 54.1400
2459 CA ASP 217 0.0245 72.0745 68.4600
2471 CA ASP 218 0.0220 69.9812 69.9300
2483 CA SER 219 0.0214 69.4673 66.9500
2494 CA LYS 220 0.0143 63.5682 63.7000
2516 CA VAL 221 0.0120 61.6214 60.2000
2532 CA PHE 222 0.0159 64.8931 50.2700
2552 CA LYS 223 0.0188 67.3324 64.4000
2574 CA GLU 224 0.0297 76.3445 73.9700
2589 CA GLY 225 0.0240 71.6606 64.1500
2596 CA SER 226 0.0165 65.4036 65.8800
2607 CA CYS 227 0.0117 61.3945 69.4400
2617 CA LEU 228 0.0087 58.8889 68.0100
2636 CA LEU 229 0.0075 57.8594 69.4600
2655 CA ALA 230 0.0106 60.4805 61.5600
2665 CA ASP 231 0.0113 61.0267 68.0600
2677 CA ASP 232 0.0110 60.7951 74.7300
2689 CA ASN 233 0.0124 61.9762 75.4600
2703 CA PHE 234 0.0089 59.0232 57.1500
2723 CA VAL 235 0.0072 57.5943 43.6300
2739 CA LEU 236 0.0093 59.4063 56.2800
2758 CA ILE 237 0.0119 61.5483 69.2800
2777 CA GLY 238 0.0079 58.1918 69.2100
2784 CA SER 239 0.0068 57.2608 69.0200
2795 CA PHE 240 0.0101 60.0126 76.9600
2815 CA VAL 241 0.0105 60.3727 74.8400
2831 CA SER 242 0.0075 57.8665 85.5000
2842 CA PHE 243 0.0067 57.1672 87.8400
2862 CA PHE 244 0.0108 60.6280 78.1300
2882 CA ILE 245 0.0133 62.7035 77.5400
2901 CA PRO 246 0.0114 61.1502 58.2700
2915 CA LEU 247 0.0109 60.7081 62.0600
2934 CA THR 248 0.0159 64.8376 70.5800
2948 CA ILE 249 0.0171 65.8685 58.9600
2967 CA MET 250 0.0145 63.7120 59.5700
2984 CA VAL 251 0.0179 66.5407 69.4100
3000 CA ILE 252 0.0231 70.8551 59.6000
3019 CA THR 253 0.0213 69.3968 58.1800
3033 CA TYR 254 0.0200 68.2993 49.5300
3054 CA PHE 255 0.0260 73.2812 52.2900
3074 CA LEU 256 0.0285 75.3414 48.6700
3093 CA THR 257 0.0256 72.9442 55.8300
3107 CA ILE 258 0.0277 74.6682 57.3100
3126 CA LYS 259 0.0336 79.6204 60.4500
3148 CA SER 260 0.0326 78.7983 57.3600
3159 CA LEU 261 0.0301 76.7148 72.3300
3178 CA GLN 262 0.0344 80.2844 77.3700
3195 CA LYS 263 0.0381 83.3972 72.3400
3217 CA GLU 264 0.0335 79.5222 87.7500
3232 CA ALA 265 0.0325 78.7314 50.6600
3242 CA ALA 1001 0.0374 82.7567 58.3100
3252 CA ASP 1002 0.0318 78.1494 68.1700
3264 CA LEU 1003 0.0225 70.3933 64.7300
3283 CA GLU 1004 0.0289 75.7491 67.5200
3298 CA ASP 1005 0.0292 75.9902 74.8700
3310 CA ASN 1006 0.0166 65.4240 61.5200
3324 CA TRP 1007 0.0162 65.1328 67.1000
3348 CA GLU 1008 0.0277 74.7038 55.4800
3363 CA THR 1009 0.0214 69.4880 54.7300
3377 CA LEU 1010 0.0117 61.3670 57.4700
3396 CA ASN 1011 0.0239 71.5604 61.6300
3410 CA ASP 1012 0.0343 80.2319 55.2700
3422 CA ASN 1013 0.0253 72.6844 61.4800
3436 CA LEU 1014 0.0289 75.6905 77.2400
3455 CA LYS 1015 0.0531 95.8409 80.6400
3477 CA VAL 1016 0.0520 94.9487 57.6800
3493 CA ILE 1017 0.0472 90.9757 73.5300
3512 CA GLU 1018 0.0681 108.3308 62.5000
3527 CA LYS 1019 0.0940 129.9739 74.0500
3549 CA ALA 1020 0.0874 124.4903 82.1600
3559 CA ASP 1021 0.1275 157.8801 85.0900
3571 CA ASN 1022 0.1165 148.7108 98.5500
3585 CA ALA 1023 0.0991 134.2209 88.4900
3595 CA ALA 1024 0.0995 134.5442 89.3300
3605 CA GLN 1025 0.0763 115.2241 94.6000
3622 CA VAL 1026 0.0568 98.9429 79.3900
3638 CA LYS 1027 0.0637 104.6641 80.3600
3660 CA ASP 1028 0.0594 101.1098 70.9900
3672 CA ALA 1029 0.0356 81.2727 73.2200
3682 CA LEU 1030 0.0328 78.9612 80.9600
3701 CA THR 1031 0.0469 90.6885 67.8800
3715 CA LYS 1032 0.0343 80.1961 77.5400
3737 CA MET 1033 0.0186 67.1305 60.9800
3754 CA ARG 1034 0.0339 79.8381 75.9100
3778 CA ALA 1035 0.0407 85.5358 66.7900
3788 CA ALA 1036 0.0233 71.0133 70.6100
3798 CA ALA 1037 0.0221 70.0001 88.6100
3808 CA LEU 1038 0.0454 89.4248 72.8700
3827 CA ASP 1039 0.0399 84.9039 90.1900
3839 CA ALA 1040 0.0292 75.9232 70.4300
3849 CA GLY 1041 0.0564 98.6312 85.3700
3856 CA ASP 1066 0.0818 119.7973 100.7900
3868 CA ILE 1067 0.0705 110.3873 101.6400
3887 CA LEU 1068 0.0440 88.3296 81.4000
3906 CA VAL 1069 0.0527 95.5623 85.9900
3922 CA GLY 1070 0.0812 119.2682 116.3700
3929 CA GLN 1071 0.0620 103.3045 96.7500
3946 CA ILE 1072 0.0434 87.7842 86.3700
3965 CA ASP 1073 0.0735 112.8357 95.6600
3977 CA ASP 1074 0.0899 126.5283 94.6400
3989 CA ALA 1075 0.0643 105.2434 80.9200
3999 CA LEU 1076 0.0708 110.6492 97.6300
4018 CA LYS 1077 0.1115 144.5229 84.9000
4040 CA LEU 1078 0.1042 138.4141 92.1200
4059 CA ALA 1079 0.0869 124.0095 79.5100
4069 CA ASN 1080 0.1212 152.6053 73.8400
4083 CA GLU 1081 0.1538 179.7788 69.9600
4098 CA GLY 1082 0.1375 166.1649 78.8300
4105 CA LYS 1083 0.1294 159.4136 70.5500
4127 CA VAL 1084 0.1017 136.3873 86.8800
4143 CA LYS 1085 0.1118 144.7732 77.5400
4165 CA GLU 1086 0.1098 143.1021 94.9900
4180 CA ALA 1087 0.0742 113.4876 67.8000
4190 CA GLN 1088 0.0669 107.3461 73.9200
4207 CA ALA 1089 0.0841 121.6653 77.3700
4217 CA ALA 1090 0.0657 106.3515 73.9500
4227 CA ALA 1091 0.0402 85.0989 77.8900
4237 CA GLU 1092 0.0537 96.3907 96.1000
4252 CA GLN 1093 0.0596 101.2879 86.0200
4269 CA LEU 1094 0.0335 79.5589 75.1800
4288 CA LYS 1095 0.0333 79.3797 86.6200
4310 CA THR 1096 0.0490 92.4706 75.2800
4324 CA THR 1097 0.0395 84.5697 66.9600
4338 CA ILE 1098 0.0202 68.4399 70.4300
4357 CA ASN 1099 0.0314 77.7914 67.6900
4371 CA ALA 1100 0.0424 86.9790 74.0300
4381 CA TYR 1101 0.0299 76.5443 59.4900
4402 CA ILE 1102 0.0221 70.0691 90.3400
4421 CA GLN 1103 0.0373 82.6796 86.6200
4438 CA LYS 1104 0.0409 85.6662 66.4600
4460 CA TYR 1105 0.0315 77.8734 74.4600
4481 CA GLY 1106 0.0312 77.6320 70.9700
4488 CA GLN 313 0.0399 84.8812 83.1500
4505 CA SER 314 0.0361 81.7238 70.2700
4516 CA ILE 315 0.0307 77.2295 84.2900
4535 CA SER 316 0.0337 79.6964 81.0600
4546 CA ASN 317 0.0306 77.1095 72.0700
4560 CA GLU 318 0.0273 74.3410 57.7000
4575 CA GLN 319 0.0270 74.1294 72.6000
4592 CA LYS 320 0.0256 72.9849 83.1400
4614 CA ALA 321 0.0218 69.7730 65.4600
4624 CA CYS 322 0.0213 69.3805 74.0200
4635 CA LYS 323 0.0207 68.8560 70.5900
4657 CA VAL 324 0.0174 66.0957 62.6900
4673 CA LEU 325 0.0145 63.7159 54.9100
4692 CA GLY 326 0.0154 64.4454 54.7700
4699 CA ILE 327 0.0130 62.4629 54.3300
4718 CA VAL 328 0.0095 59.5748 42.6800
4734 CA PHE 329 0.0099 59.8619 59.7600
4754 CA PHE 330 0.0115 61.1714 65.7800
4774 CA LEU 331 0.0081 58.3600 69.8700
4793 CA PHE 332 0.0056 56.3136 61.4100
4813 CA VAL 333 0.0075 57.8850 48.5900
4829 CA VAL 334 0.0088 58.9562 51.2900
4845 CA MET 335 0.0057 56.3470 70.6700
4862 CA TRP 336 0.0043 55.2315 72.0600
4886 CA CYS 337 0.0079 58.1770 63.5600
4897 CA PRO 338 0.0080 58.2542 71.1700
4911 CA PHE 339 0.0054 56.1460 71.1100
4931 CA PHE 340 0.0057 56.3552 76.1500
4951 CA ILE 341 0.0104 60.2758 64.9300
4970 CA THR 342 0.0097 59.7177 60.1700
4984 CA ASN 343 0.0074 57.7814 81.1100
4998 CA ILE 344 0.0094 59.4481 70.3300
5017 CA MET 345 0.0140 63.3174 77.3100
5034 CA ALA 346 0.0127 62.2002 74.5100
5044 CA VAL 347 0.0140 63.2604 69.6300
5060 CA ILE 348 0.0199 68.1667 85.1900
5079 CA CYS 349 0.0253 72.6975 102.9900
5089 CA LYS 350 0.0247 72.2374 104.3100
5111 CA GLU 351 0.0568 98.9659 91.4800
5126 CA SER 352 0.0600 101.6342 92.9200
5137 CA CYS 353 0.0351 80.8506 84.5500
5147 CA ASN 354 0.0359 81.5429 86.3000
5161 CA GLU 355 0.0250 72.4981 83.3200
5176 CA ASP 356 0.0317 78.0687 80.4600
5188 CA VAL 357 0.0265 73.7017 75.0300
5204 CA ILE 358 0.0166 65.4577 81.0500
5223 CA GLY 359 0.0152 64.2676 60.6000
5230 CA ALA 360 0.0179 66.5372 70.4100
5240 CA LEU 361 0.0133 62.7036 64.8900
5259 CA LEU 362 0.0083 58.5615 61.4700
5278 CA ASN 363 0.0089 59.0602 69.5700
5292 CA VAL 364 0.0093 59.3441 72.2100
5308 CA PHE 365 0.0062 56.8307 69.9000
5328 CA VAL 366 0.0040 54.9357 60.1600
5344 CA TRP 367 0.0042 55.0850 52.1800
5368 CA ILE 368 0.0050 55.8072 58.6800
5387 CA GLY 369 0.0031 54.2294 52.5600
5394 CA TYR 370 0.0025 53.7267 70.4800
5415 CA LEU 371 0.0038 54.8119 82.5300
5434 CA ASN 372 0.0039 54.8809 71.9200
5448 CA SER 373 0.0034 54.4300 75.3000
5459 CA ALA 374 0.0047 55.5477 61.5100
5469 CA VAL 375 0.0062 56.7760 67.7700
5485 CA ASN 376 0.0064 56.9890 73.3200
5499 CA PRO 377 0.0075 57.8625 56.9200
5513 CA LEU 378 0.0097 59.6978 63.2400
5532 CA VAL 379 0.0111 60.8968 60.5300
5548 CA TYR 380 0.0123 61.8429 61.7500
5569 CA THR 381 0.0141 63.3486 69.9200
5583 CA LEU 382 0.0179 66.5292 61.7500
5602 CA PHE 383 0.0190 67.4795 57.5700
5622 CA ASN 384 0.0201 68.3863 60.2700
5636 CA LYS 385 0.0256 72.9340 64.9900
5658 CA THR 386 0.0229 70.7087 69.4300
5672 CA TYR 387 0.0160 64.9881 56.2900
5693 CA ARG 388 0.0180 66.6268 57.3400
5717 CA SER 389 0.0227 70.5550 72.6900
5728 CA ALA 390 0.0187 67.2264 83.6700
5738 CA PHE 391 0.0154 64.4493 71.6900
5758 CA SER 392 0.0217 69.6978 87.6900
5769 CA ARG 393 0.0249 72.4132 65.5400
5793 CA TYR 394 0.0206 68.7769 81.0500
5814 CA ILE 395 0.0268 73.9669 80.0800
5833 CA GLN 396 0.0364 81.9493 71.8900
5850 CA CYS 397 0.0369 82.3682 72.1600
5861 CA GLN 398 0.0371 82.5323 99.0300
5878 CA TYR 399 0.0323 78.5036 100.5300
5899 CA LYS 400 0.0473 91.0672 108.0100
5921 CA GLU 401 0.0389 84.0728 102.8500

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: October 18th, 2018.

Should you encounter any unexpected behaviour, please let us know.