Should you encounter any unexpected behaviour,
please let us know.

* *

B-factor analysis for ID 220306205521121170

Overall B-factor prediction

Correlation= 0.585 for 214 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA GLN 24 0.2758 73.0962 78.8800
11 CA SER 25 0.2721 72.4398 79.4700
17 CA ARG 26 0.1572 52.2226 80.6300
28 CA SER 27 0.1424 49.6116 78.5600
34 CA GLU 28 0.1242 46.4136 80.6200
43 CA LEU 29 0.0807 38.7481 76.8600
51 CA GLU 30 0.0515 33.6160 73.5800
60 CA ARG 31 0.0712 37.0889 73.7000
71 CA ARG 32 0.0621 35.4773 68.1300
82 CA MET 33 0.0331 30.3696 60.4000
90 CA MET 34 0.0236 28.7017 49.8500
98 CA VAL 35 0.0479 32.9885 45.8100
105 CA GLU 36 0.0205 28.1579 35.2800
114 CA ASP 37 0.0237 28.7317 36.6600
122 CA SER 38 0.0492 33.2119 33.7900
128 CA TYR 39 0.0263 29.1785 25.1400
140 CA GLU 40 0.0146 27.1258 25.2400
149 CA ALA 41 0.0149 27.1687 22.1200
154 CA PRO 42 0.0257 29.0779 26.1100
161 CA ARG 43 0.0248 28.9186 25.0400
172 CA GLY 44 0.0223 28.4733 21.9200
176 CA ARG 45 0.0172 27.5748 20.4300
187 CA LEU 46 0.0107 26.4353 19.0900
195 CA PHE 47 0.0087 26.0887 20.7900
206 CA ASN 48 0.0251 28.9764 23.0000
214 CA GLU 49 0.0202 28.1038 20.7300
223 CA GLN 50 0.0470 32.8256 26.8500
232 CA ALA 51 0.0340 30.5367 26.3000
237 CA TYR 52 0.0160 27.3667 23.0800
249 CA LEU 53 0.0216 28.3614 26.1400
257 CA ARG 54 0.0459 32.6268 25.9200
268 CA GLY 55 0.0396 31.5300 31.4200
272 CA GLU 56 0.0435 32.2107 36.5900
281 CA THR 57 0.0287 29.6065 31.0300
288 CA TYR 58 0.0119 26.6515 28.1000
300 CA LEU 59 0.0084 26.0232 23.7400
308 CA ASN 60 0.0129 26.8192 24.4100
316 CA GLN 61 0.0120 26.6629 24.9800
325 CA TYR 62 0.0079 25.9451 20.2600
337 CA THR 63 0.0104 26.3842 22.1500
344 CA ASN 64 0.0073 25.8408 19.9100
352 CA VAL 65 0.0060 25.6021 18.5700
359 CA ILE 66 0.0049 25.4113 15.4400
367 CA VAL 67 0.0057 25.5529 16.1700
374 CA ILE 68 0.0063 25.6643 14.0800
382 CA ASN 69 0.0084 26.0347 19.2000
390 CA LYS 70 0.0098 26.2722 19.9000
399 CA ALA 71 0.0130 26.8358 21.2100
404 CA ALA 72 0.0248 28.9252 28.1400
409 CA SER 73 0.0492 33.2143 25.7100
415 CA GLY 74 0.0895 40.2985 29.5300
419 CA SER 75 0.0712 37.0834 28.4400
425 CA GLN 76 0.0216 28.3590 29.9000
434 CA ALA 77 0.0156 27.2967 26.0200
439 CA GLN 78 0.0094 26.2078 18.1600
448 CA THR 79 0.0094 26.2099 20.4100
455 CA LEU 80 0.0076 25.8938 20.6200
463 CA ARG 81 0.0076 25.8850 19.0800
474 CA LEU 82 0.0094 26.2032 18.9400
482 CA TYR 83 0.0089 26.1220 20.4000
494 CA THR 84 0.0157 27.3182 22.2300
501 CA ASN 85 0.0215 28.3329 21.6700
509 CA ARG 86 0.0215 28.3292 22.0900
520 CA GLN 87 0.0300 29.8237 21.1300
529 CA LEU 88 0.0199 28.0558 19.9200
537 CA MET 89 0.0257 29.0773 21.2800
545 CA LEU 90 0.0153 27.2502 20.2400
553 CA SER 91 0.0145 27.1074 19.0300
559 CA THR 92 0.0146 27.1235 21.9000
566 CA TYR 93 0.0112 26.5234 20.3300
578 CA VAL 94 0.0074 25.8613 19.1300
585 CA SER 95 0.0085 26.0501 20.7200
591 CA THR 96 0.0085 26.0505 21.8800
598 CA GLY 97 0.0111 26.5077 25.9200
602 CA ARG 98 0.0179 27.6996 31.0200
613 CA GLU 99 0.0147 27.1468 31.2200
622 CA ASP 100 0.0213 28.3061 39.9600
630 CA LEU 101 0.0209 28.2310 41.1500
638 CA GLU 102 0.0412 31.8057 50.5400
647 CA TYR 103 0.0633 35.6952 69.5400
659 CA ILE 104 0.1226 46.1384 71.7900
667 CA SER 105 0.3157 80.1289 83.2400
673 CA LYS 116 1.1277 223.0555 85.5900
682 CA GLY 117 0.5395 119.5169 84.5000
686 CA ALA 118 0.2853 74.7759 82.3100
691 CA THR 119 0.1240 46.3704 79.0700
698 CA SER 120 0.1205 45.7587 71.7100
704 CA SER 121 0.0500 33.3494 66.0000
710 CA HIS 122 0.0262 29.1617 49.3200
720 CA TRP 123 0.0159 27.3581 40.1200
734 CA ARG 124 0.0145 27.1035 37.5000
745 CA HIS 125 0.0124 26.7414 31.0600
755 CA THR 126 0.0091 26.1515 21.7200
762 CA THR 127 0.0094 26.2103 21.5400
769 CA ARG 128 0.0095 26.2325 20.4000
780 CA GLY 129 0.0097 26.2572 20.5200
784 CA PHE 130 0.0073 25.8295 17.0600
795 CA TYR 131 0.0082 25.9954 20.1600
807 CA THR 132 0.0082 25.9940 17.3100
814 CA ILE 133 0.0084 26.0301 19.8700
822 CA LYS 134 0.0142 27.0549 23.6100
831 CA ARG 135 0.0147 27.1348 24.8400
842 CA VAL 136 0.0134 26.9107 21.4500
849 CA HIS 137 0.0147 27.1425 26.4000
859 CA GLY 138 0.0231 28.6179 28.9000
863 CA TYR 139 0.0219 28.4086 31.0600
875 CA ASN 140 0.0364 30.9623 37.7100
883 CA TYR 141 0.0232 28.6340 38.3700
895 CA ARG 142 0.0326 30.2900 45.8500
906 CA SER 143 0.0463 32.6973 51.9900
912 CA GLY 144 0.1437 49.8506 69.8100
916 CA GLU 145 0.1366 48.6012 76.0900
925 CA SER 146 0.0765 38.0195 74.6800
931 CA LYS 147 0.0850 39.5192 76.0200
940 CA PHE 148 0.0346 30.6454 62.6300
951 CA HIS 149 0.0244 28.8467 47.2100
961 CA MET 150 0.0126 26.7709 34.8800
969 CA PRO 151 0.0117 26.6158 29.0900
976 CA PHE 152 0.0101 26.3313 28.4600
987 CA ALA 153 0.0100 26.3034 26.9800
992 CA MET 154 0.0078 25.9191 21.6100
1000 CA PHE 155 0.0096 26.2482 20.2600
1011 CA PHE 156 0.0090 26.1360 21.3100
1022 CA ASN 157 0.0111 26.5016 21.0800
1030 CA GLU 158 0.0213 28.2965 28.7200
1039 CA THR 159 0.0378 31.2131 29.5900
1046 CA ARG 160 0.0156 27.2987 29.4600
1057 CA GLY 161 0.0181 27.7381 24.5900
1061 CA LEU 162 0.0094 26.2126 21.7000
1069 CA ALA 163 0.0082 25.9958 21.7200
1074 CA VAL 164 0.0061 25.6249 21.1700
1081 CA HIS 165 0.0059 25.5872 26.6900
1091 CA GLN 166 0.0086 26.0636 25.7600
1100 CA VAL 167 0.0104 26.3742 27.3300
1107 CA PRO 168 0.0171 27.5689 35.9000
1114 CA PRO 169 0.0245 28.8604 36.8700
1121 CA ASP 170 0.0387 31.3678 42.7200
1129 CA LEU 171 0.0364 30.9526 45.1400
1137 CA SER 172 0.1314 47.6771 48.3900
1143 CA GLY 173 0.1629 53.2263 50.4200
1147 CA GLY 174 0.0599 35.1036 44.7000
1151 CA GLU 175 0.0350 30.7121 42.8900
1160 CA ALA 176 0.0578 34.7241 41.9900
1165 CA ALA 177 0.0580 34.7571 38.0900
1170 CA GLY 178 0.0243 28.8290 33.7600
1174 CA GLU 179 0.0227 28.5484 33.6200
1183 CA ALA 180 0.0397 31.5354 37.0700
1188 CA MET 181 0.0244 28.8429 33.7000
1196 CA LEU 182 0.0125 26.7509 28.3900
1204 CA GLY 183 0.0153 27.2466 23.5600
1208 CA LYS 184 0.0185 27.8049 24.3000
1217 CA ARG 185 0.0134 26.9076 24.3100
1228 CA ALA 186 0.0142 27.0429 30.2200
1233 CA SER 187 0.0135 26.9262 29.2200
1239 CA SER 188 0.0153 27.2506 34.6100
1245 CA GLY 189 0.0113 26.5397 25.8100
1249 CA CYS 190 0.0079 25.9336 22.8300
1255 CA VAL 191 0.0061 25.6197 21.8400
1262 CA ARG 192 0.0065 25.7034 22.7900
1273 CA VAL 193 0.0063 25.6537 22.1500
1280 CA HIS 194 0.0127 26.7927 24.8800
1290 CA LYS 195 0.0154 27.2622 26.8300
1299 CA ASN 196 0.0293 29.7020 27.1800
1307 CA GLN 197 0.0155 27.2843 26.7000
1316 CA ILE 198 0.0100 26.3068 26.2300
1324 CA GLN 199 0.0134 26.9020 23.8400
1333 CA THR 200 0.0152 27.2318 24.4600
1340 CA ILE 201 0.0110 26.4793 20.2000
1348 CA HIS 202 0.0120 26.6616 26.6500
1358 CA ARG 203 0.0217 28.3717 22.9600
1369 CA ALA 204 0.0148 27.1532 21.7400
1374 CA VAL 205 0.0119 26.6469 19.1000
1381 CA THR 206 0.0247 28.8961 23.8000
1388 CA ALA 207 0.0363 30.9487 26.3500
1393 CA ALA 208 0.0214 28.3161 24.2600
1398 CA ASP 209 0.0259 29.1084 31.2000
1406 CA ARG 210 0.0281 29.5066 28.3200
1417 CA GLY 211 0.0309 29.9836 26.4400
1421 CA ILE 212 0.0331 30.3711 26.6100
1429 CA VAL 213 0.0180 27.7276 24.0800
1436 CA PRO 214 0.0153 27.2365 25.9500
1443 CA VAL 215 0.0212 28.2891 20.9200
1450 CA LEU 216 0.0129 26.8295 24.1300
1458 CA ASP 217 0.0182 27.7594 25.5100
1466 CA THR 218 0.0166 27.4721 23.4200
1473 CA ARG 219 0.0202 28.0986 28.8100
1484 CA THR 220 0.0214 28.3124 28.5600
1491 CA GLY 221 0.0142 27.0569 24.5900
1495 CA GLN 222 0.0258 29.0910 24.2500
1504 CA PRO 223 0.0196 28.0063 26.1100
1511 CA LYS 224 0.0331 30.3736 26.4800
1520 CA TYR 225 0.0470 32.8282 27.3400
1532 CA ASP 226 0.1315 47.6962 27.4200
1540 CA GLN 227 0.2652 71.2301 28.4200
1549 CA ASN 228 0.2560 69.6167 30.8200
1557 CA GLY 229 0.1671 53.9593 27.8000
1561 CA LYS 230 0.0879 40.0247 33.1000
1570 CA VAL 231 0.0376 31.1670 25.3200
1577 CA ARG 232 0.0353 30.7597 28.5800
1588 CA TYR 233 0.0325 30.2774 27.1800
1600 CA GLU 234 0.0492 33.2141 25.4000
1609 CA LYS 235 0.0517 33.6502 24.8300
1618 CA GLY 236 0.0215 28.3279 22.6700
1622 CA TYR 237 0.0137 26.9559 20.1100
1634 CA LYS 238 0.0098 26.2731 21.9900
1643 CA THR 239 0.0076 25.8851 18.5300
1650 CA ILE 240 0.0054 25.4961 20.2800
1658 CA VAL 241 0.0054 25.5062 16.0200
1665 CA ILE 242 0.0056 25.5460 18.4900
1673 CA VAL 243 0.0065 25.6924 17.4500
1680 CA GLU 244 0.0098 26.2752 19.4500
1689 CA GLU 245 0.0124 26.7376 18.4100
1698 CA TYR 246 0.0211 28.2569 22.3100
1710 CA TYR 246 0.0211 28.2681 22.2800

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: October 18th, 2018.

Should you encounter any unexpected behaviour, please let us know.

*** ***

B-factor analysis for ID 220306205521121170

Overall B-factor prediction

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

Should you encounter any unexpected behaviour,
please let us know.

* *