Thursday, April 25th 2019                           
Analytical platform

Isotope ratio mass spectrometry (IRMS):

Spectrometers:

Isotope ratio mass spectrometer with 2 interfaces: elemental analyzer and Gaz Chromatography (AE/ CG-C/ SMRI Delta V Advantge Thermo Electron ).

Isotope ratio mass spectrometer with an elemental analyzer (AE/ SMRI Integra 2 Sercon).

 

Staff:

SCHIPHORST Anne-MarieOffice 043
Tel 02 51 12 57 05 /02 76 64 50 84
Anne-Marie.Schiphorst@univ-nantes.fr
GRAND MathildeOffice 014
Tel 02 51 12 57 11
mathilde.grand@univ-nantes.fr

Mass spectrometry:

The operation CPER "Spectrométrie de masse CEISAM" of the project CISPEO is cofunded by the European Union
through the funds of the region Pays de la Loire (FEDER).
Europe en Pays de la Loire

 

Spectrometers:

UPLC-MS (WATERS)

UPLC : Acquity H-Class

MS : Xevo G2-XS Qtof

Source/Probes : ESI, ESI/APCI, ASAP (Atmospheric Solid Analysis Probe)

Modes of introduction : liquid (infusion, LC-MS), solide (ASAP)

Analysis : MS, exact mass measuring (HRMS), MS/MS, LC-MS

 

 

 

GC-MS (AGILENT TECHNOLOGIES)

GC : 7890B

MS : 5977B

Source/Probe : EI (source Inert Plus self-cleaning), TSP (Thermal Separation Product)

Modes of introduction : liquid (GC ou TSP), solide (TSP)

Analysis : MS, GC-MS, Database search

 

Staff:

HEMEZ JulieBureau 042
Tél 02 76 64 51 72
02 51 12 54 08 (lab)
julie.hemez@univ-nantes.fr
ARZEL LaurenceBureau 135
Tél 02 51 12 54 92
02 51 12 54 08 (lab)
laurence.arzel@univ-nantes.fr

Operation :

The mass spectrometry platform is dedicated to the study of organic and synthetic organo-metal compounds or natural products. It is possible to realize :

  • Mass spectra ;
  • Accurate mass measurements (high resolution) ;
  • Chromatographic analyzes of complex mixtures (LC or GC-MS) ;
  • Help with structural elucidation .

The analyzes are carried out by the members of the platform. All requests for analysis must be accompanied by a completed form.

Elemental analyzis :

Device:

elemental analyzer for C, H, N, S (Flash 2000 by Thermo Scientific) .

 

Staff:

LOQUET DenisOffice 043
Tel 02 51 12 57 13
denis.loquet@univ-nantes.fr
HEMEZ JulieOffice 042
Tel 02 76 64 51 72
julie.hemez@univ-nantes.fr

Atomic Absorption Spectroscopy:

Device:

Atomic Absorption Spectrometer THERMO SCIENTIFIC ICE 3300
Flame "Air/Acetylene" or "Nitrous Oxyde/Acetylene"

Analysis with Flame Air/Acetylene:
Li, Na, Mg, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, Rb, Sr, Ru, Rh, Pd, Ag, Cd, In, Sn, Sb, Te, Cs, Ba, Ir, Pt, Au, Hg, Tl, Pb, Bi.
Available lamps in the lab : Pb, Fe/Ni, Na/K, Ca/Si, Cd/Zn, Mg, Ag, Ga, Sn, Cu et Pd.

Analysis with Flame Nitrous oxide /Acetylene :
Be, B, Al, Si, P, Ca, Sc, Ti, V, Cr, Ga, Ge, As, Se, Sr, Y, Zr, Nb, Mo, Rh, Sn, Ba, La, Hf, Ta, W, Re, Os, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Th, U.
Available lamps in the lab : Ca/Si, Ga, Sn, Gd, As P, Ho and Ti .

 

Staff:

LEFEVRE François-XavierOffice 240
Tel 02 51 12 54 05
francois-xavier.lefevre@univ-nantes.fr

ATOMIC FORCE MICROSCOPE :

Operation CPER "CEISAM Atomic Force Microscope" of the CISPEO project is cofunded by the European Union
through the funds of the region Pays de la Loire (FEDER) .
Europe en Pays de la Loire

 

Device:

http://www.sciences.univ-nantes.fr/CEISAM/pix/common/europe.png ATOMIC FORCE MICROSCOPE JPK NanoWizard®4 combined with NIKON Ti2-U inverted optical microscope .

Measuring mode :

  • Contact.
  • Dynamic.
  • Force Modulation (QI Advanced TM mode).
  • Kelvin Probe Force Microscopy (KPM mode).

 

Sample holder :

  • Temperature module (-30°C to +120°C +/- 0.1°C) designed for opaque samples (JPK HCM TM ). Sample size : 15x15 mm. .
  • Temperature module (RT to +60°C +/- 0.1°C) designed for transparent samples (PetriDishHeater TM ). Compatible with 35 mm Petri dishes.

Inverted Microscope :

Objective:

  • Nikon CFI S P-Fluor ELWD 60xC/ 0.95/WD : 0,21-0,11mm.
  • Nikon CFI S P-Fluor ELWD 40xC/ 0.95/ WD : 0,25-0,16mm.
  • Nikon CFI Plan Apochromat Lambda 20X/ 0.75/ 1,00 mm.

LED Source.

Fluorescence filters :

  • -DAPI-50LP-A.
  • -FITC-3540C.
  • -Cy3-4040C.
  • -Cy5-4040C.

Isolation:

JPK acoustic enclosure with Accurion's Halcyonics i4 acitve vibration isolation system.

 

Operation :

Le microscope à Force Atomique est adapté aux échantillons inorganiques - organiques - biologiques – sous air ou en milieu liquide.
Les mesures sont réalisées par François-Xavier LEFEVRE.

Staff :

LEFEVRE François-XavierBureau 240
Tél 02 51 12 54 05
francois-xavier.lefevre@univ-nantes.fr